Abstract
Vitamin D has been associated with many health conditions. Because of widespread deficiency in the general population, laboratory testing of vitamin D has increased exponentially in recent years. Currently, 25-hydroxyvitamin D (25[OH]D) is considered the best marker of vitamin D status. Automated immunoassays and tandem mass spectrometry are the most widely used assays for the measurement of 25(OH)D. Because a medical decision of vitamin D deficiency and treatment are made based on specific levels, it is important that different 25(OH)D assays are harmonized. Despite standardization efforts, significant differences remain among various methods and laboratories for the measurement of 25(OH)D.
